Tag Archives: wine production

Geeking Out About Grape Variety Clones

As part of the Wine Production Program at South Seattle College our instructor, Peter Bos, arranged for a private tour of the famed Red Willow Vineyard in the Yakima Valley with Mike and Jonathan Sauer back in 2012.

Photo taken by self and uploaded to Wikimedia Commons under CC-BY-SA-3.0

Mike and Jonathan Sauer of Red Willow Vineyard

In Washington State, few vineyards are as legendary and influential as Red Willow. Working with both Dr. Walter Clore and Master of Wine David Lake at Columbia Winery, the Sauers and Red Willow helped pioneer the commercial plantings of numerous grape varieties in the state. This include Viognier, Malbec, Mourvedre, Nebbiolo, Sangiovese, Tempranillo and Syrah among others.

On the trip, a question was posed to Mike Sauer about what the future focus should be for the wine industry–not just in Washington, but globally.

Without missing a beat he replied with one word–Clones.

What The Heck Are Clones?

Essentially clones are examples of grape varieties with a slight genetic mutation. These mutation could allow the vine to ripen a tad earlier, bloom a bit later, have tinier berries or thicker skin, absorb nutrients better, etc.

To best understand this, let’s take a step back to look at how grapevines are propagated in the nursery and vineyard.

Rather than plant seeds (which will produce a completely different grape variety), new vines of particular grape varieties like Chardonnay, Cabernet Sauvignon, etc are most often propagated via cuttings from an original mother vine. Here a branch with fruiting buds is removed from an active vine and then either planted to develop its own roots or, more commonly, grafted onto phylloxera resistant rootstock and planted. In theory, this new cutting is genetically identical to its mother vine.

But sometimes growers observe differences in the vineyard or the nursery among these seemingly identical vines. The clonal mutations with the most beneficial traits are selected for future propagation and eventual commercial use.

What Does This Mean For Winemakers?

Photo by scrumpyboy (Mark Shirley). Uploaded to Wikimedia Commons under CC-BY-2.0

Grapevine cuttings

Clones add another shade of color to the winemaker’s palette. Instead of just having one shade of blue (Syrah), you can plant multiple clones of Syrahs in similar terroir and end up with a multitude of shades. With these colors (Cerulean, Azure, Cobalt, etc), you can paint a deeper picture and potentially make a more complex wine.

They also allow viticulturists and winemakers to narrow in on exactly which clones perform best on different kinds of terrior, essentially following the path of the natural self-selection that we’ve seen in varieties like Sangiovese. Over centuries of time, this grape has adapted and developed its own unique clones. In the galestro clay soils of the Chianti Classico region, we have Sangioveto.  In the more limestone and schist based soils mixed with galestro in Brunello di Montalcino  produced Sangiovese Grosso. Meanwhile, the Pliocene-era sand and clay based soils of Vino Nobile di Montepulciano brought about Prugnolo Gentile.

Take one of these unique clones, plant them in different soils, and you will end up with different wines. Such is the magic and possibilities of clones.

A Few of My Favorite Resources On Clones

*Pl@ntGrape Project (yes, it is spelled with that silly ‘@’) — a joint project between several French agencies to catalog all the different grape varieties and their clones in France. When you search for a particular grape variety, at the bottom of the page is a listing of popular clones. The site also notes where they originated and their characteristics.

For example, Syrah has over 600 clones studied with 12 approved for commercial propagation. These include clones 100, 174, 300, 470, 471, 524, 525, 747, 877, 1140, 1141 and 1188

Photo by Stephan Ridgway. Uploaded to Wikimedia Commons under CC-BY-2.0

Syrah grapes growing in the Hunter Valley of Australia.

Chenin blanc has over 200 clones studied with 8 approved for commercial propagation. These include clones 220, 278, 416, 417, 624, 880, 982 and 1018.

Riesling has nearly 190 clones that have been studied with 8 approved for commercial propagation. These include clones 49, 1089, 1090, 1091, 1092, 1094, 1096 and 1097.

Malbec (Cot) has around 220 clones that have been studied with 16 approved for commercial propagation. These include clones 42, 46, 180, 279, 353, 419, 592, 593, 594, 595, 596, 597, 598, 1061, 1127 and 1128.

*UC Davis Foundation Plant Services page on Pinot noir probably has the most extensive listing (in English) and description of Pinot noir clones I’ve found. This is pretty impressive since Pinot noir is known to have over a 1000 different clones.

*The Science of Grapevines: Anatomy and Physiology by Markus Keller. There is some hardcore geeking here with this viticulture textbook. It not only covers clones but also the science behind how the mutations happened. It also goes into the broad spectrum of grapevine anatomy and physiology. These factors, like disease resistance, nutrient utilization, etc. plays into the decisions of how different clones are selected.

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What’s fine (and not so fine) about Vegan Wines

Photo by www.Pixel.la Free Stock Photos. Uploaded to Wikimedia Commons under CC-Zero

Veganism is described as one of the fastest growing lifestyle movements in the world. Some estimates claim that in the United states alone, there was a 500% increase between 2014 and 2017 in the number of Americans (around 19 million) identifying as vegan.

For the wine industry, that is a sizable demographic that retailers and wineries have value in marketing to.

So what makes a wine “vegan-friendly”?

For the most part, veganism is a code of conduct that avoids using any animal products or by products as well as anything that has been tested on animals. There are various reasons why people adopt veganism but often ethical concerns about the treatment of animals and impact on the environment are cited.

While wine is often assumed to be vegan, the use of animal-based fining agents such as casein (milk protein), albumin (egg whites), isinglass (fish bladders) and gelatin (animal collagen) in winemaking is problematic for many vegans.

Let’s Talk About Fining Agents

As Alison Crowe notes in The Wine Maker’s Answer Book, fining agents are used to help clarify and stabilize wine by binding to molecules such as proteins and excess tannins. These are items that can cause unsightly haze in the bottle, aggressive bitterness on the palate, off odors and flavors. The agent binds to the target molecule to form larger structures that eventually precipitates and settles to the bottom of tank or barrel as sediment.

Bruce W. Zoecklein et. al in Wine Analysis and Production classified the various fining agents into 8 categories based on their nature.

Photo by self. Uploaded as User:Agne27 to Wikimedia Commons under CC-BY-SA-3.0

Isinglass and bentonite fining trials.

1.) Earths like bentonite and kaolin
2.) Proteins like the animal based ones above
3.) Polysaccharides like gum arabic and Sparkolloid
4.) Carbons like activated carbon
5.) Synthetic polymers like polyvinylpolypyrrolidone (or PVPP)
6.) Silica gels like silicon dioxide or Kieselsol
7.) Tannins often derived from insect galls on oak leaves though oak chip fining can also fall into this category.
8.) Others which includes both enzymatic fining (more fining aids rather than fining agents) and chelators that assist in the removal of metals such as “blue fining” with potassium ferrocyanide (illegal to use in the United States).

The different fining agents work on principles of electrical charge (like positively charged gelatin reacting with negatively charged tannins), bond formation (like the carbonyl group of PVPP bonding with the hydroxyl group of tannins) and by absorption/adsorption (like activated carbon absorbing off odors or bentonite adsorbing proteins).

There are positive and negative attributes to each fining agent with no one fining agent being perfect for every situation.

Animal-based Fining Agents

Egg whites (Albumin)

Used primarily to remove excess tannins. Works by forming hydrogen bonds with the hydroxyl groups of tannins. Compared to other fining agents like gelatin, albumin tends to remove less positive flavor and aroma traits. Egg whites have a long history of use in winemaking in places like Bordeaux and Burgundy. The usual addition is 1 to 3 egg whites per 225L (59 gallon) barrel.

Casein (Milk protein)

Used primarily to remove browning or pink color in white wine. Can also be used to remove some off odors. Works by adsorption and attracting negatively charged particles. Like with egg whites, it has a long history of use in wine production, particularly with the great white wines of Burgundy. It also has the benefit of reducing the concentration of iron and copper in wine. In red wines, it can negatively impact the wine by removing the polyphenol resveratrol that has been associated with various health benefits.

Gelatin (derived from the boiling of animal tissues like bones and tendons)

Used primarily to remove excess tannins. It has a positive charge that reacts to the negative charge of harsh tannins. It can be prone to over-fining that can strip a wine of positive flavors and aromas.

A heat stability trial for rose wines that have been fined with isinglass.

Isinglass (derived from the air bladder of fish like sturgeons)

Used primarily to help clarifying wines, remove excess tannins and to “unmask” or bring out varietal character.

Chitosan (derived from chitin in the exoskeleton of crustaceans)

Used primarily to remove haze causing proteins from white wines. A positively charged agent, it often needs to be paired with a negatively charged fining agent like Kieselsol to be most effective.

Blood Albumen (derived from the blood of ox and cattle)

Historically used but illegal in the United states, France and several other countries.

Vegan-friendly Alternatives

The website Barnivore is a database of wines and other liquors that have been vetted by users to be either “vegan-friendly” or not. In answering queries about their use of animal based fining agents, many wineries share their alternative methods.

Photo by self. Uploaded as User:Agne27 to Wikimedia Commons under CC-BY-SA-3.0

Letting the wine settle and clarify on its own before racking into another container is one method to avoid using fining agents.

One common method is the use of time and gravity to let the wine settle and clear out on its own. This is the technique used by Baldacci in the Stags Leap District and many other wineries. Depending on several factors like the health of the grapes, method of pressing, pH and temperature, this method could take several months and even then the wine might not be completely stable. Some wineries facilitate this method with the use of mechanical centrifuges and ultra-filtration but these carry the risk of being overly aggressive and potentially stripping the wine of positive flavor and aroma attributes.

Along those lines, many wineries adopt a hands-off method of not fining or filtering their wines at all. This is the method used by many high-end wine producers like Black Cordon and Kapcsandy in Napa Valley. This does carry the risk of haze and sediment developing in the bottle. However, the risk is often presented to consumers as a trade-off for having potentially more complex and flavorful wines.

Bentonite and Yeast Fining
Photo by self. Uploaded as User:Agne27 to Wikimedia Commons under CC-BY-SA-3.0

The lees sediment and volume loss from bentonite can be significant (between 5-10%). Using counter-fining agents like isinglass can help with lees compaction but would obviously make the wine not vegan-friendly.

The most used “vegan-friendly” fining agent is bentonite. This is a type of clay that can dramatically swell in size to adsorb protein molecules. This is the method used by wineries like Chinook in Washington State, Ideology in Napa Valley, Spier in South Africa and many others. One big drawback is that it causes significant loss of wine volume due to the heavy sediment it creates. As much as 5-10% of volume could be lost. Roger B. Boulton et al notes in Principles and Practices of Winemaking that these voluminous bentonite lees also create a large amount of solid waste that can have an environmental impact (such as sealing percolation ponds) if not properly disposed. In red wine, there is also a risk of color loss.

Some wineries like Amici use the technique of “yeast fining” for wines like their 2013 Russian River Pinot noir. This involves adding fresh yeast to a wine.  The cell walls of the yeast contain about 30% positively charged proteins. These can then adsorb many polyphenols and compounds which cause off odors. It brings the risk of the yeast breaking down as lees, releasing sulfur compounds and enhancing reductive notes. Also, if not removed by filtration, the yeast in the bottle can start re-fermenting any residual sugars. This would cause spritziness in what is, otherwise, supposed to be a non-sparkling wine.

PVPP and Other Methods

PVPP is a synthetic polymer that can remove bitter tannins and brown discoloration from white wines. Like casein, it can remove the polyphenol resveratrol from red wines. There is also a risk of overfining. This is because the PVPP also binds to desirable tannins and anthocyanins needed for structure and color.

Sparkalloid is a blend of polysaccharides and diatomaceous earth (fossilized algae) that can be used to clarify white wines. It does take significant time to eventually settle. This also creates a fair amount of waste with the DE that requires proper disposal.

Activated Carbon can be used to remove off-odors such as mercaptans (rotten eggs, burnt match). It does have the risk of overly oxidizing wine as well as stripping color and resveratrol from red wines.

New Developments on the Horizon

Ronald S. Jackson notes in Wine Science that fears about the prions potentially in gelatin and “Mad Cow” disease, encouraged studies into the use of plant proteins like wheat gluten as a substitute for gelatin. (Note: most gelatin used in US winemaking is derived from pigs rather than cows) Likewise, a New Jersey company has been experimenting with using pea proteins in conjunction with bentonite and silica as an alternative to gelatin.

Interest in food allergies have also spurned innovations with Scott Labs developing a technique to isolated chitosan from the fungus Aspergillus niger (instead of shellfish and crustaceans) that can be used as a fining agent.

The California based ATP Group has developed a way to extract tannin powder for fining from white wine grapes instead of insect galls to help soften tannins.

In 2016, a Swiss company announced that they were experimenting with the use of UV light to soften tannins in lieu of using animal-based protein fining agents.

The Biodynamic Quandary

Are wines produced from fruit sourced from biodynamic vineyards truly “vegan-friendly”? Several of the “preparations” used in biodynamic viticulture require the use of animal products such as cow horns (BD 500 and 501), stomachs, intestines and bladders.

In an anecdotal account of a visit with the vineyard manager of the biodynamic Pinot noir producer Sea Smoke, Kirsten Georgi (The Armchair Sommelier), describes how the “Biodynamic approach” to removing gophers without the use of poisons or chemicals involves trapping several gophers, killing them, burning their ashes and spreading those ashes over the vineyard during winter solstice as a means to “scare off” the rest of the gophers. This method of “peppering” vineyards with the ashes of pests is not unique to Sea Smoke with recipes on biodynamic websites recommending its use for everything from weeds, snails and insects to mice, rats, rabbits and opossum.

PETA Approved?
Photo by Mark Smith. Uploaded to Wikimedia Commons under CC-BY-2.0

Manure composting at a biodynamic vineyard in Tasmania.

Despite these practices, organizations like PETA recommend biodynamic wineries as “vegan friendly”. The UK website Vegan Wines Online notes that while “…natural animal products can however be used in the growing process all the biodynamic wines they sell are somehow suitable for vegans.

Even organic viticulture could be problematic with the use of animal-derived bone and blood meal being used in lieu of chemical fertilizers. There is even debate if manure, as an animal by-product, is acceptable. Like honey and milk, manure doesn’t require killing the animal but still often requires farming to acquire.

What about the presence of insects in healthy and vibrant organic vineyards? Does that makes a wine less “Vegan-friendly”? Eschewing the use of chemicals often means more insects as MOG (material other than grapes). Absent expensive sorting these bugs can get caught up in the harvest. On the Barnivore website, Calcareous Vineyard in Paso Robles expressed this reservation though their wines were still classified as “vegan friendly”.

More Manipulated=More Vegan-friendly?

Are the most “vegan-friendly” vineyards the ones being farmed with heavy saturation of pesticides and chemical fertilizers? It seems like it when you compare it to organic and biodynamic vineyards with high insect MOG and animal-derived fertilizers.

Mass produced wines like the PETA recommended Sutter Home and Moët & Chandon often employ these conventional, chemical dependent styles of viticulture.

While avoiding using animal-based fining agents to remove excess tannins and haze forming proteins, big mega-corps can use other tricks to manipulate the wine with things like lab designed enzymes, oak adjuncts and Mega Purple which will “smooth out” bitter tannins and cover up off-flavors.

Just a single drop of Mega Purple had this white Riesling looking and smelling like a Grenache rose. Crazy stuff.

Sure, Charles Shaw reds (Two Buck Chuck), Sutter Home Cabernet Sauvignon, Meiomi Pinot noir and Yellow Tail reds are made without animal fining agents but should vegans (and really all wine drinkers) be concerned with what other products are being used to make these wines?

And while it can be exciting to see advances in the use of pea proteins and fining agents derived from fungi like Aspergillus niger, its worth asking if these are only adding to the laundry list of the 60+ (and counting) additives that can be used in winemaking–taking it even further away from being just “fermented grapes”?

Now What?

While I’m not vegan myself, I wholeheartedly support anyone that chooses to live their life by convictions. I respect their ethical concerns for the treatment of animals.  I also share their concerns about the environment animal products have. It’s not my wish to stress-out vegans who just want to relax and enjoy a nice bottle of wine.

I do believe it is fair to think about the big picture involved in many seemingly “vegan-friendly” wineries.  Often the viticulture and winemaking practices they use may not align with the ideals of many vegans.

However, it is clear from sites like Barnivore that there are tons of environmentally conscious wineries (many of which are even owned by vegans) that are producing vegan-friendly wines. They may not be the easiest to find at grocery stores or restaurant wine lists that can be dominated by the portfolios of the large mega-corps but these often small family-owned wineries are well worth seeking out and supporting.

And that’s something that I think both vegans and non-vegans can drink to.

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